D-3-羟基丁酸脱氢酶(EC 1.1.1.30),是一种以NAD+或NADP+为受体、作用于供体CH-OH基团上的氧化还原酶。此酶催化D-3-羟基丁酸反应,而D-3-羟基丁酸是血酮体中主要成分,可用于酶法诊断血酮体增高引起糖尿病酮症酸中毒。
Appearance Yellowish powder, lyophilized
Source Microorganism
Enzyme Commission Number EC 1.1.1.30
CAS Number 9028-38-0
Storage temperature -20℃
Specific activity ≥1500U/mg protein
Unit definition One unit converts one micromole of 3-Hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.
PROPERTIES
Molecular weight 27.5 kDa (SDS-PAGE)
Isoelectric point 7.25
Optimum pH: 8.0
Optimum temperature: 6 ℃
pH Stability 7.0~10.0 (25℃, 2 hr)
Thermal stability < 37℃ (pH 8.5, 30min)